K5 Polysaccharide

High Purity Guarantee with Stable Quality: Purity >98%, prepared from E. coli 010:K5:H4 and purified via proprietary process to ensure consistent performance in experiments.·Clear Structural Advantage, Suitable for Multi-Enzymatic Research: Backbone composed of [→4)-β-D-glucuronic acid (GlcA)-(1→4)-N-acetyl-α-D-glucosamine (GlcNAc)-(1→]n repeating disaccharide unit, structurally similar to heparin and heparan sulfate.·Diverse Substrate Applications, Meeting Multiple Research Needs: Serves as a substrate for K5 lyase A (Cat#: E-KFLA) and N-deacetylase/N-sulfotransferase (NDST) enzyme, applicable to heparin biosynthesis and polysaccharide degradation studies.·Reliable Detection Support, Facilitating Experimental Analysis: Degraded by K5 lyase A into octa-, hexa-, tetra-, and disaccharide with Δ4,5GlcA at the non-reducing end, detectable by UV spectroscopy at 232 nm.For laboratory use only www.carbomenu.com

Product Details

K5 Polysaccharide

Synonyms: Polysaccharide K5, K5 heparosan, capsular polysaccharide K5| CAS: 42615-44-1 | Source: E. coli 010:K5:H4 | Average Molecular Weight: ~50,000-70,000 Da |K5 Polysaccharide, also known as K5 heparosan, is a capsular polysaccharide produced by the E. coli K5 strain, bearing a backbone composed of a [→4)-β-D-glucuronic acid (GlcA)-(1→4)-N-acetyl-α-D-glucosamine (GlcNAc)-(1→]n repeating disaccharide unit.

Because of its structural similarity to heparin and heparan sulfate—biologically important molecules involved in blood anticoagulation, bacterial and viral infection, and other physiological and pathophysiological events—K5 polysaccharide offers a promising precursor for preparation of a bioengineered heparin using an enzymatic combinatorial approach, and generation of all kinds of substrates for the enzymes involved in the biosynthesis of heparin and heparan sulfate.

As a specific substrate, K5 polysaccharide can be degraded by K5 lyase A (Cat#: E-KFLA) into octa-, hexa-, tetra-, and disaccharide with Δ4,5GlcA at the non-reducing end which can be detected by UV spectroscopy at 232 nm. Meanwhile, it can also be used as a substrate for the N-deacetylase/N-sulfotransferase (NDST) enzyme. The high quality size-defined K5 polysaccharide is prepared from E. coli 010:K5:H4, and purified using our proprietary process.

HPGPC analysis of K5 polysaccharide
The ¹H-NMR spectrum of K5 polysaccharide
The ¹³C-NMR spectrum of K5 Polysaccharide
Identification of K5 polysaccharide (K5PS) with K5 lyase A (KflA)